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Oxford, UK – The trimeric HIV type 1 (HIV-1) envelope glycoprotein (Env) is targeted by broadly neutralizing antibodies (bNAbs) produced by the immune system during infection. As part of Ludger’s collaboration with Dr Max Crispin and his group at the University of Oxford we performed site-specific N-glycosylation analysis of the gp120 and three gp41 subunits of Env.
Using MALDI-MS, LC-MS and HILIC-UPLC our results uncovered a dominance of oligomannose-type glycans and revealed a mosaic of glycan microclusters bearing under-processed glycans, especially in areas covering the gp120 outer domain and at the trimer interfaces. The information gained from this study will assist in the design of Env-based vaccine immunogens and the work has now been published in Cell Reports :
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